KPL researchers publish BioRxiv preprint demonstrating superior performance of our Lys-C enzyme for proteomics applications

22 October 2024

Company News | Products

COPENHAGEN, Denmark, October 22, 2024 – KPL ApS, a pioneering company focused on advancing proteomics and protein engineering, announces the publication of groundbreaking research validating its Lys-C as the highest-performing enzyme for mass spectrometry-based proteomics. The comprehensive study, conducted in collaboration with researchers from the prestigious Novo Nordisk Foundation Center for Protein Research at the University of Copenhagen, presents the first systematic comparison of Lys-C enzymes originating from different bacterial species and demonstrates KPL’s enzyme’s exceptional performance across multiple critical parameters.

Key Performance Highlights:

Industry-Leading Cleavage Efficiency: KPL’s Lys-C achieved an unprecedented >80% cleavage efficiency in early digestion time points, reaching >90% of peptides with zero missed cleavages after overnight digestion – significantly outperforming competitors that achieved only 70% efficiency at best.

Superior Peptide Identification: Consistently identified more peptides across all time points, particularly excelling in shorter digestion times, leading to enhanced protein coverage and more comprehensive proteome analysis.

Exceptional Protein Coverage: Demonstrated superior sequence coverage of proteins, with A. lyticus Lys-C identifying the highest number of unique proteins and achieving the most comprehensive coverage with fully cleaved peptides.

Enhanced Reproducibility: Exhibited superior consistency with lower coefficient of variation (CV) values across replicates, ensuring reliable and reproducible results crucial for quantitative proteomics research. – Optimal Trypsin Combination: When combined with trypsin, KPL’s LysC significantly improved digestion efficiency, achieving ~85% zero-missed-cleavage peptides compared to just 70% with trypsin alone. This improvement was particularly pronounced for lysine-containing peptides, where trypsin alone showed approximately 30% missed cleavages.

Versatile Performance: Maintained stable performance in denaturing conditions up to 3M urea and 2M guanidine hydrochloride, offering flexibility in various experimental conditions. The study also revealed a linear relationship between enzyme concentration and digestion time, allowing researchers to optimize their workflows by adjusting these parameters.

Reliability for Proteomic Workflows:

The research, now available on BioRxiv, utilized state-of-the-art protein aggregation capture (PAC) workflow with HeLa cell lysate to comprehensively assess enzyme performance across various experimental conditions. The study evaluated multiple parameters including cleavage specificity, digestion efficiency, and performance reproducibility.

“This landmark study validates what our customers have experienced – that our Lys-C enzyme sets the new gold standard for proteomics applications,” -Cristina Hernández Rollán , CTO KPL ApS.

“The superior performance of our Lys-C, particularly in reducing missed cleavages and improving peptide identification, makes it an essential tool for researchers seeking maximum efficiency and reliability in their proteomics workflows.” – Tanveer Singh Batth, CEO KPL ApS.

Why This Matters:

The study confirms that KPL’s Lys-C represents the optimal choice for proteomics researchers, offering unmatched performance in both standalone applications and in combination with trypsin. These findings are particularly significant for laboratories conducting high-throughput proteomics research where efficiency and reliability are paramount.

Key links:

Read the BioRxiv paper here: https://www.biorxiv.org/content/10.1101/2024.10.18.619105v1

To purchase our Lys-C: https://kplbio.com/product/lysc/