Arg-C Zero
Arginyl endopeptidases (Arg-C) cleave specifically at the C-terminus of Arginine residues. Our novel sequencing grade Arg-C Zero™ is a high-fidelity enzyme that can achieve close to 0% missed cleavage rates. It is the perfect protease for many proteomic applications. Originally identified as a virulence factor from Porphyromonas gingivalis, Arg-C Zero delivers complete digestion of your protein mixture, ensuring reproducibility and accurate results across experiments. Arg-C Zero is produced recombinantly using Escherichia coli to high purity and compatible for mass spectrometry-based proteomic analysis.
Product Details
Arg-C Zero [KPL0146] is a sequencing-grade Arginyl endopeptidase that cleaves specifically at the C-terminus of Arginine residues. Engineered for high fidelity, Arg-C Zero delivers nearly 0% missed cleavage rates, making it ideal for a wide range of proteomic applications.
Originally identified as a virulence factor from Porphyromonas gingivalis, Arg-C Zero ensures complete digestion of protein mixtures, leading to reproducibility and accurate results across multiple experiments. Produced recombinantly in Escherichia coli, Arg-C Zero achieves high purity and is fully compatible with mass spectrometry-based proteomics analysis.
Experimental conditions: Digestion of HeLa protein extract. Arg-C Zero to total protein ratio of 1:50 in 50mM HEPES pH 8.5 buffer, 5mM DTT. Sample digested overnight at 37°C and analyzed using LC/MS/MS.
Technical Specifications
Formulation
Liquid or lyophilized
Denaturing Conditions
Retains high activity in up to 4M Urea.
pH Range
6 – 10
Enzyme-to-Protein Ratio
Recommended Arg-C Zero to total protein ratio 1:50. Low ratios such as 1:500 can also be used while maintaining high digestion efficiency.
Recommended Digestion Buffer
HEPES or PBS buffer with 5mM reducing agent TCEP (Tris [2-carboxyethyl] phosphine) or DTT (Dithiothreitol).
A reducing agent is recommended for efficient activity.
Note: The buffer must not contain alkylating agents such as Iodoacetic acid (IAA), Iodoacetamide (IAM), or Chloroacetamide (CAA).
More Technical Specifications
| Property | Specification |
|---|---|
| Product Name | Arg-C Zero |
| Product Number | KPL0146 |
| Specificity | Cleaves at the C-terminus of Arginine residues |
| Formulations | Liquid, Lyophilized |
| Optimal pH Range | 6 – 10 |
| Buffer Compatibility | HEPES buffer, 5mM TCEP or DTT (no alkylating agents) |
| Denaturing Conditions | Retains activity in up to 4M Urea |
| Enzyme-to-Protein Ratio | 1:50 |
| Quality Control | Stringent QC under typical proteomics experimental conditions |
| Source | Recombinantly produced in Escherichia coli |
| Applications | Mass spectrometry-based proteomic analysis, in-solution digestion, SP3/PAC workflows. |
| Safety Data Sheet |
Quality Control
Each batch of Arg-C Zero [KPL0146] undergoes stringent quality control under experimental conditions representative of typical proteomics experiments. This ensures that the product delivers consistent results across various use cases.
Key Features of Arg-C Zero
High Fidelity
Achieves close to 0% missed cleavage rates
PTM analysis
Ideal for analysis of elusive PTMs such as histone modifications.
Recombinant Production
Produced in Escherichia coli to high purity.
Mass Spectrometry Compatible
Optimized for proteomics analysis.
Complete Digestion
Ensures reproducibility and accuracy in protein mixture digestion.
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Protocols
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References
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FAQ
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