Lys-C
Lysyl endopeptidases (Lys-C) cleave specifically at the C-terminus of Lysine residues. Our recombinant sequencing grade Lys-C is produced in Escherichia coli to high purity, compatible for mass spectrometry-based proteomics analysis. Originally identified in Achromobacter lyticus, our Lys-C outperforms all alternatives by consistently delivering precision in peptide cleavage and minimizing missed cleavages. This superior cleavage efficiency ensures reproducibility and accurate results across experiments. With our Lys-C, researchers can confidently push the boundaries of discovery, unlocking a deeper understanding of complex proteomes with unprecedented reliability.
Product Details
Our Lys-C [KPL0033] is available as a liquid or lyophilized formulation. It is compatible with different sample preparation protocols and digestion buffers such as Tris-HCl and HEPES. The recommended pH range for our Lys-C is between pH 7-9. Our Lys-C retains high activity under high denaturing conditions up to 8M Urea and 2M Guanidine Chloride. We perform stringent quality control on our Lys-C with experimental conditions representative of typical proteomics experiments to guarantee consistent results across batches
Experimental conditions: 10µg HeLa digest, PAC sample clean-up using magnetic beads. Lys-C to protein ratio of 1:100 in 50mM HEPES pH 8.5 buffer. Sample digested overnight at 37°C and analyzed using LC/MS.
Technical Specifications
Formulation
Liquid or lyophilized
Compatible Buffers
Tris-HCl, HEPES
pH Range
7-9
High Activity Conditions
Up to 4M Urea and 2M Guanidine Chloride
Quality Control
Stringent testing under typical proteomics experimental conditions
More Technical Specifications
| Property | Specification |
|---|---|
| Product Name | Lys-C |
| Specificity | Cleaves at the C-terminus of Lysine residues |
| Formulations | Liquid, Lyophilized |
| Optimal pH Range | 7 – 9 |
| Buffer Compatibility | Tris-HCl, HEPES |
| Denaturing Conditions | Retains activity in up to 8M Urea and 2M Guanidine Chloride |
| Enzyme-to-Protein Ratio | 1:100 (as per experimental conditions provided) |
| Quality Control | Stringent QC under typical proteomics experimental conditions |
| Source | Recombinantly produced in Escherichia coli |
| Applications | Mass spectrometry-based proteomic analysis, various sample preparation protocols |
| Safety Data Sheet |
Quality Control
Each batch of Lys-C [KPL0033] undergoes stringent quality control under experimental conditions representative of typical proteomics experiments. This guarantees consistent results across different batches and applications.
Key Benefits of Using Lys-C
Superior Precision in Peptide Cleavage
Our Lys-C consistently delivers precision in peptide cleavage, ensuring accurate experimental results.
Minimizes Missed Cleavages
With superior cleavage efficiency, our Lys-C minimizes missed cleavages, providing more reliable data.
Ensures Reproducibility and Accurate Results
Stringent quality control ensures that Lys-C offers reproducibility and accuracy across all experiments.
Compatibility with Mass Spectrometry-Based Proteomics
Lys-C is specifically designed to be compatible with mass spectrometry-based proteomics, enhancing research outcomes.
High Activity Under Denaturing Conditions
Our Lys-C retains high activity even under high denaturing conditions, making it versatile for various experimental setups.
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