Trypsin RMS
Recombinant, sequencing-grade trypsin based on the Sus scrofa sequence. Produced in E. coli for superior purity, stability, and reproducibility in mass spectrometry-based proteomics.
✓ Reductively methylated
✓ MS-compatible
✓ High purity
Product Details
Trypsin RMS [KPL0208] is a sequencing-grade serine protease that cleaves specifically at the C-terminus of Lysine and Arginine residues. Based on the Sus scrofa trypsin sequence, it delivers the familiar cleavage characteristics proteomics labs rely on, while [R]ecombinant production in Escherichia coli ensures that animal-derived contaminants such as Chymotrypsin are eliminated from the final product. Homogenous reductive [M]ethylation of all lysine residues dramatically reduces autolytic degradation, ensuring exceptional stability throughout digestion workflows and extended experimental conditions. [S]equencing-grade purity ensures our Trypsin RMS delivers the precision and reliability demanded by the most rigorous proteomics workflows.
With high catalytic efficiency and robust performance across varied experimental conditions, Trypsin RMS outperforms traditional animal-derived alternatives while maintaining consistent activity over extended digestion periods. Recombinant production eliminates the batch-to-batch variability inherent to tissue extraction, delivering consistently high purity. Trypsin RMS is the ideal workhorse for mass spectrometry-based proteomics applications requiring the highest level of reproducibility and performance.
Experimental conditions: HeLa digest, Trypsin RMS to total protein ratio of 1:50 in 50mM HEPES pH 8.5 buffer was used. Samples were digested overnight at 37°C and analyzed using LC/MS/MS.
Technical Specifications
Formulation
Liquid or lyophilized
Compatible Buffers
Tris-HCl, Ammonium Bicarbonate, HEPES, PBS, TEAB, and other standard buffers.
pH Range
Optimal between pH 7 – 9. Active at pH 5 – 7 with lower efficiency.
Enzyme-to-Protein Ratio
Recommended Trypsin RMS to total protein ratio 1:50 or 1:25 for optimal digestion depending on sample type.
Methylated
Trypsin RMS is reductively methylated at Lysine residues to limit auto-proteolysis and increase stability.
High specificity
Recombinant production eliminates co-purifying contaminants such as Chymotrypsin, ensuring strict cleavage specificity at C-terminus of Arginine and Lysine residues only.
More Technical Specifications
| Property | Specification |
|---|---|
| Product Name | Trypsin RMS |
| Product Number | KPL0208 |
| Specificity | Cleaves at the C-terminus of Arginine and Lysine residues |
| Formulations | Liquid, Lyophilized |
| Optimal pH Range | 7 – 10 |
| Buffer Compatibility | Tris-HCl, Ammonium Bicarbonate, HEPES, PBS, TEAB |
| Enzyme-to-Protein Ratio | 1:50 |
| Quality Control | Stringent QC under typical proteomics experimental conditions |
| Source | Recombinantly produced in Escherichia coli |
| Applications | Mass spectrometry-based proteomic analysis, in-solution digestion, in-gel digestion, FASP, SP3/PAC workflows. |
| Safety Data Sheet |
Quality Control
Each batch of Trypsin RMS [KPL0208] undergoes stringent quality control under experimental conditions representative of typical proteomics experiments. This ensures that the product delivers consistent results across various use cases.
Key Features of Trypsin RMS
Batch-to-batch consistency
Every lot performs identically, eliminating variability that can compromise quantitative proteomics and longitudinal studies.
Wide Application Range
Digest single proteins or complex samples such as cell lines, tissues, Plasma, PTMs and more. Trypsin RMS is compatible across various proteomic applications.
Recombinant Production
Produced in Escherichia coli to sequencing grade quality for mass spectrometry-based proteomics.
Sequencing grade
Optimized for bottom-up proteomics at high purity with a clean formulation that won’t introduce contaminants or ion suppression into your MS analysis.
Optimized for automation
Reliable performance across liquid handling platforms and high-throughput sample preparation workflows.
Produced sustainably
Trypsin RMS delivers premium digestion performance without any animal-derived components, contributing to sustainable practices.
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Frequently Asked Questions (FAQ)
1. What is Trypsin RMS?
Trypsin RMS is a recombinant, sequencing-grade serine protease designed for mass spectrometry-based proteomics. It cleaves specifically at the C-terminus of lysine and arginine residues.
2. Why is it called “RMS”?
RMS stands for [R]ecombinantly produced, (reductively) [M]ethylated, [S]equencing-grade—reflecting the homogeneous methylation of lysine residues that minimizes autolysis and its suitability for demanding proteomics applications.
3. What organism is Trypsin RMS derived from?
Trypsin RMS is based on the Sus scrofa (porcine) trypsin sequence but is produced recombinantly in Escherichia coli, combining familiar cleavage characteristics with animal-free production.
4. How does recombinant production benefit my experiments?
Recombinant production eliminates animal-derived contaminants like Chymotrypsin and removes the batch-to-batch variability inherent to tissue extraction, ensuring consistent performance across experiments.
5. Is Trypsin RMS compatible with LC-MS workflows?
Yes. Trypsin RMS is formulated without detergents or additives that interfere with mass spectrometry, allowing direct analysis with minimal sample cleanup.
6. What does reductive methylation do?
Methylation of lysine residues blocks Trypsin’s self-cleavage sites, dramatically reducing autolysis, increase stability while maintaining enzyme activity throughout extended digestions.
7. What applications is Trypsin RMS suitable for?
Trypsin RMS is ideal for bottom-up proteomics, including protein identification, quantitative proteomics, biomarker discovery, clinical sample analysis, and high-throughput automated workflows.
8. How should I store Trypsin RMS?
Store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles by aliquoting upon receipt.
9. What digestion conditions do you recommend?
We recommend digestion at 37°C in a standard buffers such as Tris-HCl, Ammonium Bicarbonate, HEPES, TEAB, or PBS within a pH range of 7.8–8.5 and with an enzyme-to-substrate ratio between 1:20 and 1:50. Detailed protocols are available on request.
10. Is Trypsin RMS suitable for regulated or clinical research environments?
Yes. Its animal-free origin and consistent manufacturing process simplify compliance documentation for GMP settings and clinical research applications.
Protocols
Coming soon!
References
Coming soon!