Trypsin/Lys-C Premium Mix
The Trypsin/Lys-C Premium Mix delivers a ready-to-use, pre-optimized enzyme formulation that eliminates the need for separate enzyme handling and sequential digestion steps. Simply add the mix directly to your samples: no separate reconstitution, no ratio calculations, no multi-step protocols. Our high quality recombinant, animal-free Trypsin RMS and sequencing-grade Lys-C are formulated at the optimal ratio for synergistic cleavage at Lysine and Arginine residues, delivering the lowest missed cleavage rates in its class. Both enzymes are produced in Escherichia coli to the highest purity standards, ensuring complete compatibility with mass spectrometry-based proteomics workflows. The result is deeper proteome coverage, superior peptide identification, and highly reproducible results, all in a single-incubation workflow that saves time and reduces sample prep complexity.
Product Details
Our Trypsin/Lys-C Premium Mix [KPL0209] combines sequencing-grade recombinant Trypsin RMS and Lys-C in an optimized ratio for maximum digestion efficiency. Both enzymes are produced recombinantly in Escherichia coli, guaranteeing a fully animal-free formulation ideal for clinical and research applications requiring lot-to-lot consistency. Lys-C's robust cleavage at Lysine residues complements Trypsin's dual Lys/Arg specificity, together they ensure fewer missed cleavages, greater sequence coverage, and improved peptide yields in a single digestion step. Compatible with standard proteomics buffers at pH 6-10. Each batch undergoes stringent quality control under experimental conditions representative of typical proteomics experiments, guaranteeing consistent performance across batches.
Experimental conditions: HeLa digest, Trypsin/Lys-C Premium mix added at a protease to protein ratio of 1:50 in 50mM HEPES pH 8.5 buffer. Samples were digested overnight at 37°C and analyzed using LC/MS/MS.
Technical Specifications
Formulation
Lyophilized only.
Compatible Buffers
Tris-HCl, Ammonium Bicarbonate, HEPES, PBS, TEAB, and other standard buffers
pH Range
Optimal at pH 6-10.
Enzyme-to-Protein Ratio
Recommended Trypsin RMS to total protein ratio 1:50 or 1:25 for optimal digestion depending on sample type.
High specificity
Strict cleavage specificity at C-terminus of Arginine and Lysine residues only.
Optimal Ratio
Optimal ratio of Trypsin and LysC enables low missed cleavge rates, increased protein identifications, and high reproducibility.
More Technical Specifications
| Property | Specification |
|---|---|
| Product Name | Trypsin/Lys-C Premium Mix |
| Specificity | Cleaves at the C-terminus of Lysine and Arginine residues |
| Formulations | Lyophilized |
| Optimal pH Range | 6 - 10 |
| Buffer Compatibility | Tris-HCl, Ammonium Bicarbonate, HEPES, PBS, TEAB, and other standard buffers |
| Denaturing Conditions | Retains activity in up to 4M Urea and 2M Guanidine Chloride |
| Enzyme-to-Protein Ratio | 1:50 (as per experimental conditions provided) |
| Quality Control | Stringent QC under typical proteomics experimental conditions |
| Source | Both Trypsin and LysC are recombinantly produced in Escherichia coli |
| Applications | Mass spectrometry-based proteomic analysis, various sample preparation protocols |
| Safety Data Sheet |
Quality Control
Each batch of Trypsin/Lys-C Premium Mix [KPL0209] undergoes stringent quality control under experimental conditions representative of typical proteomics experiments. This guarantees consistent results across different batches and applications.
Key Benefits of Using Trypsin/Lys-C Premium Mix
Ready-to-Use Convenience
Pre-optimized enzyme ratio eliminates separate reconstitution, ratio calculations, and multi-step digestion protocols.
Recombinant Production
Both enzymes produced in Escherichia coli to sequencing-grade purity, free of animal-derived contaminants.
High Cleavage Efficiency
Delivers the lowest missed cleavage rates in its class through synergistic Lys-C and Trypsin activity.
Wide application range
Analyze cell lines, tissues, plasma, PTMs, affinity pulldowns and more. Trypsin RMS is compatible across various proteomic applications.
Optimized for automation
Compatible across liquid handling platforms and high-throughput sample preparation workflows.
Batch-to-batch consistency
Every lot performs identically, eliminating variability that can compromise quantitative proteomics and longitudinal studies.
Order now!
Order more to save more! Our Trypsin/Lys-C Premium Mix [KPL0209] is available in different volume and weight ranges for your needs.
Frequently Asked Questions (FAQ)
1. What is the Trypsin/Lys-C Premium Mix?
The Trypsin/Lys-C Premium Mix is a pre-optimized blend of our recombinant Trypsin RMS and sequencing-grade Lys-C, formulated at the optimal ratio for synergistic protein digestion in mass spectrometry-based proteomics workflows.
2. What are the advantages of using a premix over adding the enzymes separately?
The premix eliminates the need for separate reconstitution, ratio calculations, and sequential digestion steps. Simply add the mix directly to your samples for a streamlined, single-incubation workflow that delivers reproducible results with less hands-on time.
3. What is the ratio of Trypsin to Lys-C in the mix?
The enzymes are formulated at an optimized ratio based on extensive testing to maximize cleavage efficiency and minimize missed cleavages. The exact ratio is proprietary but has been validated to outperform standard Trypsin-only digestion protocols.
4. Do I still need to perform sequential digestion with this product?
No. Unlike traditional Lys-C/Trypsin protocols that require adding Lys-C first under denaturing conditions followed by dilution and Trypsin addition, the Premium Mix is designed for simultaneous single-step digestion without sacrificing performance.
5. What formulations are available?
The Trypsin/LysC Premium Mix is available as a lyophilized formulation.
6. What buffers are compatible with the Trypsin/Lys-C Premium Mix?
The mix is compatible with common proteomics buffers including Tris-HCl, HEPES, Ammonium Bicarbonate, TEAB, and PBS. The recommended pH range is 7 – 9. Trypsin/Lys-C Premium Mix is active outside this pH range but might require optimization of total protease to protein ratio.
7. Can the mix be used under denaturing conditions?
Yes. The Lys-C component retains activity in up to 4M Urea, allowing digestion of proteins that require denaturants for solubilization. For optimal Trypsin activity, we recommend diluting to ≤2M Urea before adding the mix.
8. What enzyme-to-protein ratio do you recommend?
We recommend a 1:25 to 1:50 (w/w) total enzyme-to-protein ratio for overnight digestion at 37°C. The optimal ratio may vary depending on sample type and complexity.
9. Is the Trypsin/LysC Premium Mix compatible with LC-MS workflows?
Yes. Both enzymes are recombinantly produced in Escherichia coli to sequencing-grade purity, ensuring a clean formulation free of animal-derived contaminants that could interfere with mass spectrometry analysis.
10. What applications is the mix suitable for?
The Trypsin/LysC Premium Mix is ideal for bottom-up proteomics including protein identification, quantitative proteomics, biomarker discovery, TMT/iTRAQ labeling workflows, and high-throughput sample preparation.
11. How should I store the Trypsin/Lys-C Premium Mix?
Store lyophilized product at -20°C. Once reconstituted, aliquot and store at -20°C to avoid repeated freeze-thaw cycles. Avoid keeping the reconstitute enzymes at room temperature or above for prolonged periods.
12. How do you ensure batch-to-batch consistency?
Each batch undergoes stringent quality control under experimental conditions representative of typical proteomics workflows, guaranteeing consistent digestion performance across batches.
Protocols
Coming soon!
References
Coming soon!